The majority of Czechs don’t like, or are not accustomed to, seeing same sex affection in public. Recently the government approved same sex marriage but still it is recommended keep those relations discreet. On the other hand there’s quite a few gay bars and nightclubs where you can feel comfortable. For more info about Prague Gay and Lesbian scene check amigo.cz (bi monthly magazine) or gaynet.com. There's also printed publications for lesbians called "Alia" and "Promluv".